ISO INTERNATIONAL STANDARD 16654 First edition 2001-05-01 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Escherichia coli O157 Microbiologie des aliments Methode horizontale pour la recherche des Escherichia coli 0157 Reference number ISO 16654:2001(E) ISo @ ISO 2001 py IHS under itted without license from IHS Not for Resale ISO 16654:2001(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by isO member bodies. In the unlikely event ?ISO2001 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.ch Web www.iso.ch Printed in Switzerland @ IsO 2001 - All rights reserved ermittedwithout license from IHS Not for Resale ISO 16654:2001(E) Contents Page Foreword. Introduction 1 Scope. 2 Normative references .. 3 Term and definition ... 4 Principle... 2 5 Culture media, reagents and antisera . 6 Apparatus and glassware 7 Sampling... .8 8 Preparation of test sample.. 9 Procedure (see annex A). .8 9.1 Test portion and initial suspension. .8 9.2 Enrichment.. 9.3 Immunomagnetic separation (IMs)... 9.4 Plating out onto selective agars and identification of E. coli O157 colonies.. 9 9.5 Confirmation. .10 9.6 Further characterization. 11 10 Quality assurance. 11 10.1 Test strains for quality assurance purposes. 10.2 Culture method . 11 Expression of results ... 11 12 Test report... 11 Annex A (normative) Diagram of procedure 12 Bibliography.. ii by IHS under nse from IHS Not for Resale