ISO INTERNATIONAL STANDARD 16541 Firstedition 2015-07-15 Methods for sea lice surveillance on marine finfish farms Méthodes de surveillance des poux de mer dans les exploitations de pisciculture marine Reference number IS0 16541:2015(E) ISO International Organization for Standardization Zhejiang Institute of Standardization 5956617 IS02015 Not for Resale, 2015/9/22 08:50:27 itted without license from IHS IS0 16541:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2015, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org ganizationforStandardization Not for Resale, 2015/9/22 08:50:27 etworking permitted without license from IHS IS0 16541:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. .1 2 Normative references ..1 3 Terms and definitions .1 4 Sampling design .3 4.1 Purpose, precision, and accuracy 3 4.2 Specification of measure to be used (abundance, prevalence, and median intensity) .3 4.2.1 General. 3 4.2.2 Abundance .3 4.2.3 Prevalenceandmedianintensity 4 4.2.4 Minimum requirements for on-farm monitoring of sea lice levels and effectoftreatment 4 5 Information requirements for sampling events .5 5.1 General .5 5.2 Contextual elements at each sampling event .5 5.3 Sea lice elements at each sampling event .5 5.3.1 Sea lice dislodged during handling .6 5.4 Structured format for reporting .6 6 Monitoring programme elements .7 6.1 General, .7 6.2 Selection of fish and handling. .7 6.3 Frequency for regular monitoring .7 6.4 Frequency for assessment of treatment efficacy .8 Training requirements .8 Annex A (informative) Rationale for collecting site and cage-level data 10 Annex B (informative) Typical measures used to summarize sea lice data and their variability...11 iii Institute of Standardization 5956617 thout license from IHS