ISO INTERNATIONAL STANDARD 16000-21 First edition 2013-12-15 Indoor air . Part 21: Detection and enumeration of moulds Sampling from materials Air intérieur- Partie 21: Detection et dénombrement des moisissures - Echantillonnage a partir de matériaux Reference number IS0 16000-21:2013(E) LSO IS02013 IHS under ted without license from IHS Not for Resale IS0 16000-21:2013(E) COPYRIGHTPROTECTEDDOCUMENT ISO2013 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ IS0 2013 - All rights reserved No reproductic networking permited without license from IHS Not for Resale IS0 16000-21:2013(E) Contents Page Foreword ..iv Introduction. ...vi 1 Scope. 2 Normative references 3 Terms and definitions 4 Principle of method .2 5 Apparatus and materials. .2 5.1 Equipment for sampling .2 5.2 Equipment for preparing the agar plates. .3 5.3 Equipment for processing the bulk samples .3 6 Culture media and reagents 3 6.1 General.. .3 6.2 Dichlorane 18 % glycerol agar (DG-18) .3 6.3 Maltextractagar .4 6.4 Potato dextrose agar 4 6.5 Dilution buffer. .5 6.6 Staining solution. .5 7 Measurement procedure. .6 7.1 Sampling from surfaces .6 7.2 Bulk sampling .7 7.3 Transport and storage .7 7.4 Direct microscopy 1 7.5 Suspension of material and swab samples .7 8 Quality assurance ..8 9 Sampling protocol ..8 10 Performance characteristics ..8 Annex A (informative) Sample exchange for method validation .9 Bibliography .11 iii ded by IHS under lic cense with ISt ed without license from IHS Not for Resale