论文标题
基于Kretschmann配置的表面等离子体共振生物传感器：基本仪器和应用
Surface plasmon resonance biosensors based on Kretschmann configuration: basic instrumentation and applications
论文作者
论文摘要
表面等离子体共振（SPR）为生物分子相互作用的无标签和非侵入性表征提供了强大的工具。迄今为止，已经开发了几种基于两种基本物理现象的实验构型，例如减弱的总反射和衍射，以测量由于金属表面上入射光和等离子体波之间的共振相互作用而产生的SPR信号。这些配置分为三类：基于光栅，基于棱柱和基于波导的耦合。在此类技术中，基于棱镜的SPR耦合方案之一，通常称为Kretschmann配置，由于其高灵敏度，操作简单性，较低的成本和实时检测而被最广泛使用。本章解释了基本仪器，并回顾了基于Kretschmann配置的SPR生物传感器的最新趋势。
Surface Plasmon Resonance (SPR) offers a powerful tool for label-free and non-invasive characterization of biomolecular interactions. To date, several experimental configurations, based on two fundamental physical phenomena, e.g., attenuated total reflection and diffraction, have been developed to measure the SPR signal generated due to the resonant interactions between incident light and plasma waves on the metal surface. These configurations are divided into three categories: grating-based, prism-based, and waveguide-based coupling. Among such techniques, one of the prism-based SPR coupling schemes, popularly known as Kretschmann configuration, is most widely used due to its high sensitivity, operational simplicity, lower cost, and real-time detection. This chapter explains the basic instrumentation and reviews the recent trends in the development of Kretschmann configuration-based SPR biosensors with its applications.
