论文标题
一个开源软件包，用于实用灯光灯表显微镜数据的现场直播和现场观看
An open-source software package for on-the-fly deskewing and live viewing of volumetric lightsheet microscopy data
论文作者
论文摘要
斜面显微镜OPM是一种灯表显微镜的形式，可以在高时空和空间分辨率下对生物样品进行体积成像。但是，OPM的成像几何形状以及光板显微镜的相关变体会扭曲提出的图像截面的坐标框架相对于实际空间坐标框架，其中样品被移动以导航到感兴趣的区域。这使得这种显微镜的实时观看和实用操作变得困难。我们提出了一个开源软件包，该软件包利用GPU加速和多处理来实时转换OPM成像数据的显示，以产生模仿标准广场显微镜产生的实时视图。可以以多个Hz的速率获取，处理和绘制图像堆栈，从而使OPM的实时操作以及类似的显微镜进行实时操作，更加用户友好和直观。
Oblique plane microscopy, OPM, is a form of lightsheet microscopy that permits volumetric imaging of biological samples at high temporal and spatial resolution. However, the imaging geometry of OPM, and related variants of light sheet microscopy, distorts the coordinate frame of the presented image sections with respect to real space coordinate frame in which the sample is moved to navigate to regions of interest. This makes live viewing and practical operation of such microscopes difficult. We present an open-source software package that utilises GPU acceleration and multiprocessing to transform the display of OPM imaging data in real time to produce live views that mimic that produced by standard widefield microscopes. Image stacks can be acquired, processed and plotted at rates of several Hz, making live operation of OPMs, and similar microscopes, more user friendly and intuitive.
