论文标题
测量标准比色杯中浊度样品的绝对光学特性的方法
Method for Measuring Absolute Optical Properties of Turbid Samples in a Standard Cuvette
论文作者
论文摘要
许多应用程序试图测量样品的吸收系数光谱以检索化学构成。许多现实世界的样本在光学上浑浊，导致许多商业光谱仪无法解决的散射混杂。使用扩散理论并考虑在每毫米0.01和每毫米0.01和1下的吸收和减少散射系数，我们开发了一种方法，该方法利用频域来测量标准隔板中浊度样品的绝对光学特性（45 mm（45 mm x 45 mm x 10 mm x 10 mm））。受自校准方法的启发，该方法消除了仪器混杂的方法，该方法在两组两个不同的源检测器距离的两组中使用了弥漫性复合物透射率的测量。我们发现：这最适合高度散射样品（降低的散射系数高于每毫米1）；与散射系数降低相比，吸收系数的相对误差更高。准确性与知识的折射率指数有关。具有0.1％振幅和0.1度（1.7 mrad）相的噪声模拟在吸收中发现误差和散射系数降低4％和1％。我们预计，通过高度散射样品可以减轻吸收系数的较高误差，并且通过设计具有高折射率的比色杯，可以抑制边界条件的混淆。进一步的工作将调查实施和可重复性。
Many applications seek to measure a sample's absorption coefficient spectrum to retrieve the chemical makeup. Many real world samples are optically turbid, causing scattering confounds which many commercial spectrometers cannot address. Using diffusion theory and considering absorption and reduced scattering coefficients on the order of 0.01 per mm and 1 per mm, respectively, we develop a method which utilizes frequency-domain to measure absolute optical properties of turbid samples in a standard cuvette (45 mm by 10 mm by 10 mm). Inspired by the self calibrating method, which removes instrumental confounds, the method uses measurements of the diffuse complex transmittance at two sets of two different source-detector distances. We find: this works best for highly scattering samples (reduced scattering coefficient above 1 per mm); higher relative error in the absorption coefficient compared to the reduced scattering coefficient; accuracy is tied to knowledge of the samples index of refraction. Noise simulations with 0.1 percent amplitude and 0.1 deg (1.7 mrad) phase uncertainty find errors in absorption and reduced scattering coefficients of 4 percent and 1 percent, respectively. We expect that higher error in the absorption coefficient can be alleviated with highly scattering samples and that boundary condition confounds may be suppressed by designing a cuvette with high index of refraction. Further work will investigate implementation and reproducibility.
