论文标题
藏红花中的体外微磷酸和占地去的基因表达
In vitro micropropagation and apocarotenoid gene expression in saffron
论文作者
论文摘要
藏红花（Crocus sativus L.）是一种三倍体，无菌的单子植物，属于硅藻科系列，亚果辣根。 c.sativus每年只开花一次，应在很短的时间内收集，手动收集藏红花花的污名并进行干燥，然后用作香料。它也被用作治疗肿瘤，癌症，慢性子宫出血，失眠，猩红热，天花，感冒和心血管疾病的药物。已经表明，藏红花是防止染色体损伤的保护剂。藏红花已经被CORM繁殖，每个母亲每年都会产生7-8个Cormlet。 The main colors of saffron, crocetin, and crocetin glycosides, and the main flavors, picrocrocin, Safranal is the main component of aroma and Its bitter taste is related to Glycosidepicrocrocin that is derived from the oxidative cleavage of the carotenoid, zeaxanthin.by zeaxanthin cleavage dioxygenase (ZCD).我们通过使用从伊朗霍拉桑省的Ghaen的未成熟植物芽芽获得的花生的组织培养在体外研究了ZCD的基因表达，并在伊朗省的Ghaen buds上获得了10 mg/l NAA和BAP的补充的MS培养基。使用RNX方法提取每个样品的RNA；其次是RT-PCR技术。结果表明，ZCD存在于上述区域的所有培养样品的花生中。对控制花生中藏红花阿片类素化学色素的这一途径的研究对于产生高质量和数量的藏红花很重要。结果表明，无论是通过甲丙酸还是非杂种酸，ZCD在花生中的表达都需要更多的研究
Saffron (Crocus sativus L.) is a triploid, sterile, monocot plant belonging to the family Iridaceae, sub-family Crocoideae. C.sativus only blooms once a year and should be collected within a very short duration, the stigmas of Saffron flowers are harvested manually and subjected to desiccation then have been used as a spice. It has been also used as a drug to treat tumors, cancer, chronic uterine hemorrhage, insomnia, scarlet fever, smallpox, colds, and cardiovascular disorders. It has been shown that saffron is a protective agent against chromosomal damage. Saffron has been vegetatively propagated by corm, each mother corm produces 7-8 cormlets each year. The main colors of saffron, crocetin, and crocetin glycosides, and the main flavors, picrocrocin, Safranal is the main component of aroma and Its bitter taste is related to Glycosidepicrocrocin that is derived from the oxidative cleavage of the carotenoid, zeaxanthin.by zeaxanthin cleavage dioxygenase (ZCD). We investigated gene expression of ZCD in vitro by using tissue culture of perianth obtained from immature flora buds of Ghaen of Khorasan province, Iran, on MS medium supplemented by 10 mg/L NAA and BAP. RNA of each sample was extracted using RNx method; followed by RT-PCR techniques. The results indicated that ZCD was present in perianth of all cultured samples of mentioned areas. Investigation of this pathway which controls the saffron apocarotenoid pigments in perianth is important to produce saffron with high quality and quantity. The results showed that the expression of ZCD in perianth whether through mevalonic acid or non-mevalonic acid needs more investigation
