论文标题
在几何限制的微动物中形成图案的完整收缩反应扩散模型
A full contraction-reaction-diffusion model for pattern formation in geometrically confined microtissues
论文作者
论文摘要
反应扩散模型已广泛应用，以解释基于由人类多能干细胞组成的几何密闭微动物的早期胚胎发生中的模式形成机理。最近，已经发现机械提示，例如细胞应力和菌株，决定了人类干细胞分化中的模式形成。结果，通过添加机械相关的术语来考虑机械提示所起的作用来修改传统的反应扩散模型。但是，这些模型要么不考虑细胞组织的活性，要么忽略了其毛弹性，即生物组织是由细胞和间质液制成的。因此，当前模型由于缺乏生物物理相关性而受到影响。在这里，我们提出了一个改良的反应扩散模型，该模型将其与细胞组织的主动收缩相结合。细胞组织被建模为一块双相毛弹性材料，其中机械力自然调节化学提示的运输。这种化学提示直接细胞命运，因此产生了先前实验中观察到的某些类型的模式形成。
The reaction-diffusion models have been extensively applied to explain the mechanism of pattern formations in early embryogenesis based on geometrically confined microtissues consisting of human pluripotent stem cells. Recently, mechanical cues, such as the cellular stresses and strains, have been found to dictate the pattern formation in human stem cell differentiation. As a result, the traditional reaction-diffusion models are modified by adding mechanically related terms to consider the role played by the mechanical cues. However, these models either do not consider the activeness of the cellular tissues or neglect their poroelastic nature that biological tissues are made by both cells and interstitial fluid. Hence, the current models suffer from the lacks of biophysical relevance. Here we propose a modified reaction-diffusion model that couples with the active contraction of cellular tissues. The cellular tissue is modelled as a piece of biphasic poroelastic material, where mechanical forces naturally regulate the transport of chemical cues. Such chemical cues direct cell fate and hence yield certain types of pattern formations observed in previous experiments.
