论文标题
资源分配确定了双性遗传开关中的替代细胞命运
Resource allocation determines alternate cell fate in Bistable Genetic Switch
论文作者
论文摘要
活细胞需要某些资源的持续可用性才能具有持续的基因表达过程。基因表达的细胞资源的可用性有限，例如核糖体，以及资源亲和力的变化，可以显着修改系统动力学。诸如结合速率变化或招募资源效率变化的因素具有影响关键的动力学现象，例如细胞命运的确定。在本文中，我们采用了一个非常重要的基序，即一个可动的遗传切换开关，并探讨了该电路中资源不平衡的影响。我们表明，通过资源亲和力或基因拷贝数对资源的初始不对称偏置，在干草叉和马鞍节点类型分叉中都显着修改了细胞命运转变。我们的研究确定，在有限的资源环境中，受控资源分配可能是合成或细胞遗传开关的鲁棒功能的重要因素。
Living cells need a constant availability of certain resources to have a sustained gene expression process. Limited availability of cellular resources for gene expression, like ribosomes, along with a variation of resource affinity, significantly modifies the system dynamics. Factors like the variation in rate of binding, or variation in efficiency of the recruited resource have the potential to affect crucial dynamical phenomena like cell fate determination. In this paper, we have taken a very important motif, a bistable genetic toggle switch, and explored the effect of resource imbalance in this circuit in terms of the bifurcations taking place. We show that initial asymmetric biasing to resource via resource affinity or gene copy number, significantly modifies the cell fate transition, both in pitchfork and saddle node type bifurcation. Our study establishes that in a limited resource environment, controlled resource allocation can be an important factor for robust functioning of the synthetic or cellular genetic switches.
