论文标题
多组分合金的抗微生物特性
Anti-microbial properties of a multi-component alloy
论文作者
论文摘要
诸如门把手，台面和扶手之类的高交通触摸表面可以是病原体传播的传输点，强调需要开发积极自行化的材料。由于金属的耐用性，经常用于这些表面，但是许多金属也具有通过多种机制起作用的抗菌特性。这项工作研究了由几种生物活性金属组成的金属合金，其目标是通过协同活性实现广谱，快速生物活性。提出了熵动机稳定范式，以制备可扩展的铜，银，镍和钴的合金。使用组合溅射，在100毫米晶片上制备薄膜合金，每个元素在整个晶片中的组成分级为50％。然后对电影进行退火并进行调查以保持合金稳定性。使用四种微生物 - Phi6，MS2，枯草芽孢杆菌和大肠杆菌作为人类病毒和细菌病原体的替代物，对生长的合金和退火膜进行了生物活性测试。测试表明，在与某些测试合金接触30 s后，Phi6是一种包裹的，单链的RNA噬菌体，用作SARS-COV 2替代物，最高为6.9个数量级（> 99.99999％）。此外，非发达的双链DNA噬菌体MS2，革兰氏阴性大肠杆菌和革兰氏阳性二氧化芽孢杆菌细菌菌株分别在30、20、20和10分钟后的活动中显示了5.0、6.4和5.7日志。合金样品中的生物活性表现出对组合物的强烈依赖性，而对数还原缩放直接与CU含量相比。在某些样品中退火改善活性后，通过相位分离浓度。结果促使各种主题可以利用这些主题设计理想的生物活性表面。
High traffic touch surfaces such as doorknobs, countertops, and handrails can be transmission points for the spread of pathogens, emphasizing the need to develop materials that actively self-sanitize. Metals are frequently used for these surfaces due to their durability, but many metals also possess antimicrobial properties which function through a variety of mechanisms. This work investigates metallic alloys comprised of several bioactive metals with the target of achieving broad-spectrum, rapid bioactivity through synergistic activity. An entropy-motivated stabilization paradigm is proposed to prepare scalable alloys of copper, silver, nickel and cobalt. Using combinatorial sputtering, thin-film alloys were prepared on 100 mm wafers with 50% compositional grading of each element across the wafer. The films were then annealed and investigated for alloy stability. Bioactivity testing was performed on both the as-grown alloys and the annealed films using four microorganisms -- Phi6, MS2, Bacillus subtilis and Escherichia coli -- as surrogates for human viral and bacterial pathogens. Testing showed that after 30 s of contact with some of the test alloys, Phi6, an enveloped, single-stranded RNA bacteriophage that serves as a SARS-CoV 2 surrogate, was reduced up to 6.9 orders of magnitude (>99.9999%). Additionally, the non-enveloped, double-stranded DNA bacteriophage MS2, and the Gram-negative E. coli and Gram-positive B. subtilis bacterial strains showed a 5.0, 6.4, and 5.7 log reduction in activity after 30, 20 and 10 minutes, respectively. Bioactivity in the alloy samples showed a strong dependence on the composition, with the log reduction scaling directly with the Cu content. Concentration of Cu by phase separation after annealing improved activity in some of the samples. The results motivate a variety of themes which can be leveraged to design ideal bioactive surfaces.
