论文标题
两光子显微镜中的无传感器波前校正在不同的浊度尺度上
Sensorless wavefront correction in two-photon microscopy across different turbidity scales
论文作者
论文摘要
自适应光学器件(AO)是增加多光子扫描显微镜成像深度的强大工具。对于高度散射的组织,无传感器波前校正技术表现出强大的性能,并呈现AO的直接实现。但是,对于许多应用,例如实时成像,畸变校正的速度仍然是关键的瓶颈。动态自适应散射补偿全息(DASH) - 最近在非线性扫描显微镜中引入的快速传感器AO技术 - 解决此问题。 DASH已针对高度浑浊的介质,但迄今为止,它仍然是一个空旷的问题,其在轻度浊度中的性能如何,在这种情况下,仅相位波前形状施加的局限性预计会阻碍其收敛性。在这项工作中,我们在模拟和实验中研究了跨不同浊度状态的仪表板的性能。我们进一步提供了仪表板与连续顺序算法(CSA)的新颖,修改版本之间的直接比较,我们称之为Ampliedified CSA(A-CSA)。
Adaptive optics (AO) is a powerful tool to increase the imaging depth of multiphoton scanning microscopes. For highly scattering tissues, sensorless wavefront correction techniques exhibit robust performance and present a straight-forward implementation of AO. However, for many applications such as live-tissue imaging, the speed of aberration correction remains a critical bottleneck. Dynamic Adaptive Scattering compensation Holography (DASH) -- a fast-converging sensorless AO technique introduced recently for scatter compensation in nonlinear scanning microscopy -- addresses this issue. DASH has been targeted at highly turbid media, but to-date it has remained an open question how it performs for mild turbidity, where limitations imposed by phase-only wavefront shaping are expected to impede its convergence. In this work, we study the performance of DASH across different turbidity regimes, in simulation as well as experiments. We further provide a direct comparison between DASH and a novel, modified version of the Continuous Sequential Algorithm (CSA) which we call Amplified CSA (a-CSA).