论文标题
毫克质量计量学用于定量沉积液体样品
Milligram mass metrology for quantitative deposition of liquid samples
论文作者
论文摘要
随着样品体积的减少，质量对液体的定量分配变得越来越困难。尽管可以直接称量ML液体在分析平衡或使用Pycnometer方法上进行权衡，但由于其毫克尺度的质量和快速蒸发，μL样品更具挑战性。在这里，提出了一种定量测定1μl液滴液体质量的方法。微毛细血管的称重结合了可追溯的校准质量和样品蒸发的校正，可以在1毫克的阶段确定液滴质量，而相对组合的扩张不确定性为0.3％。
Quantitative dispensing of liquids by mass becomes increasingly difficult as sample volume decreases. Whereas ml quantities of liquid can be directly weighed as dispensed on an analytical balance or using pycnometer methods, μl samples are more challenging due to their milligram-scale mass and rapid evaporation. Here, a method for quantitative determination of the mass of 1 μl droplets of liquid is proposed. The weighing of a microcapillary pycnometer combined with traceable calibration masses and corrections for sample evaporation allows determination of the droplet mass on the order of 1 mg with relative combined expanded uncertainty of 0.3 %.
