论文标题
分散剂对油滴细菌定殖的影响：微流体方法
Effect of dispersants on bacterial colonization of oil droplets: a microfluidic approach
论文作者
论文摘要
不混溶油的细菌生物降解需要细胞滴头遇到，表面附着和碳氢化合物代谢。化学分散剂被应用于漏油物以减少平均分散的液滴尺寸，从而增加可用的表面积以供附着，以促进细菌生物降解。但是，由于研究表明分散物可以抑制，增强或对生物降解没有影响，因此它们的有效性仍然存在争议。因此，关于分散物是否会影响表面附着或细胞活力的问题。在这里，使用微流体和延时显微镜，我们直接观察海洋细菌的依恋和生长，\ emph {alcanivorax borkumensis}在固定的原油滴（$ 5 $ \ textmu m $ <r <r <r <r <r <150 $ \ textmu m）的情况下，在Core core core core corne consriative nime compor nime。附件和生长取决于液滴的大小，主要由扩散相遇驱动。我们的结果表明，分散剂不会抑制或增强这些生物物理过程。
Bacteria biodegradation of immiscible oil requires cell-droplet encounters, surface attachment, and hydrocarbon metabolism. Chemical dispersants are applied to oil spills to reduce the mean dispersed droplet size, thereby increasing the available surface area for attachment, in attempts to facilitate bacterial biodegradation. However, their effectiveness remains contentious as studies have shown that dispersants can inhibit, enhance, or have no effect on biodegradation. Therefore, questions remain on whether dispersants affect surface attachment or cell viability. Here, using microfluidics and time-lapse microscopy, we directly observe the attachment and growth of the marine bacterium, \emph{Alcanivorax borkumensis}, on stationary crude oil droplets ($5$ \textmu m $< R < 150$ \textmu m) in the presence of Corexit 9500. We show that the average colonization time, or the time comprised of encounters, attachment, and growth, is dependent on droplet size and primarily driven by diffusive encounters. Our results suggest that dispersants do not inhibit or enhance these biophysical processes.
