论文标题
核中染色质的中尺度相分离
Mesoscale phase separation of chromatin in the nucleus
论文作者
论文摘要
果蝇幼虫的完整有机成像揭示并量化了染色质 - 水相分离。染色质可以在核包膜的椎板层附近组织，常规地填充核，中央组织或作为润湿液滴。这些过渡受核体积的变化以及核周围层（核包膜的一部分）的相互作用的控制。使用一个简单的聚合模型，该模型包括染色质自动吸收的关键特征及其与层的结合，从理论上讲，我们证明了这两种效应决定了染色质分布的模式。在我们的模拟中获得的定性趋势以及所获得的组成曲线与观察到的完整有机成像和定量相比。由于模拟仅包含少数物理变量，因此我们可以识别观察到的相距变化的基础机制。
Intact-organism imaging of Drosophila larvae reveals and quantifies chromatin-aqueous phase separation. The chromatin can be organized near the lamina layer of the nuclear envelope, conventionally fill the nucleus, be organized centrally, or as a wetting droplet. These transitions are controlled by changes in nuclear volume and the interaction of chromatin with the lamina (part of the nuclear envelope) at the nuclear periphery. Using a simple polymeric model that includes the key features of chromatin self-attraction and its binding to the lamina, we demonstrate theoretically that it is the competition of these two effects that determines the mode of chromatin distribution. The qualitative trends as well as the compositional profiles obtained in our simulations compare well with the observed intact-organism imaging and quantification. Since the simulations contain only a small number of physical variables we can identify the generic mechanisms underlying the changes in the observed phase separations.
