论文标题
精确的纳米化，具有高动态范围全息图
Precise nanosizing with high dynamic range holography
论文作者
论文摘要
光学传感是现代诊断的主要功能之一。尤其是无标签的成像方式，在分析之前消除了标签程序，因此具有巨大的希望。但是，纳米颗粒的散射信号及其体积方尺度。这种不利的缩放使得非常难以定量地表征本质上异质的临床样品，例如细胞外囊泡，因为它们的信号变化很容易超过当前可用的相机的动态范围。在这里，我们介绍了避开此限制的离轴k空间全息图。通过成像显微镜的后焦平面，我们将所有粒子的散射信号投射到所有相机像素上，从而显着将可实现的动态范围提高到最高110 dB。我们通过检测和定量尺寸，金属和介电颗粒在200x200 $ $ $ m $ m的视野中验证我们的平台，并证明独立执行的信号校准允许正确尺寸由不同的材料制成。最后，我们提出了细胞外囊泡样品的定量尺寸分布。
Optical sensing is one of the key-enablers of modern diagnostics. Especially label-free imaging modalities hold great promise as they eliminate labeling procedures prior to analysis. However, scattering signals of nanometric particles scale with their volume-square. This unfavorable scaling makes it extremely difficult to quantitatively characterize intrinsically heterogeneous clinical samples, such as extracellular vesicles, as their signal variation easily exceeds the dynamic range of currently available cameras. Here, we introduce off-axis k-space holography that circumvents this limitation. By imaging the back-focal-plane of our microscope we project the scattering signal of all particles onto all camera pixels thus dramatically boosting the achievable dynamic range to up-to 110 dB. We validate our platform by detecting, and quantitatively sizing, metallic and dielectric particles over a 200x200 $μ$m field-of-view and demonstrate that independently performed signal calibrations allow correctly sizing particles made from different materials. Finally, we present quantitative size-distributions of extracellular vesicle samples.
