论文标题
通过粒子特征介电摄取反应实现的细胞的高吞吐量分离并进一步聚焦
High Throughput Separation of Cells Achieved Through the Particle Characteristic Dielectrophoretic Response and Further Focusing
论文作者
论文摘要
可以在流速和粒子浓度分离的限制中限制了由于电介粒细胞的特征力而分离细胞的设备。为了释放实验室芯片技术的潜力以低成本创建灵活，高效和多功能设备，必须提高执行分离的速率。在这里，我们提出了一种能够从微粒中进行高吞吐量连续分离的装置，并在10^8-10^9细胞/ml的样品中，在微通道中显示7 ul/min的分离。该设备使用由于介电性物质引起的颗粒的特征响应，以提供初始分离，然后将颗粒关注到完全独立的粒子流中，从而使高流速和颗粒浓度以高度运行。该设备通过聚苯乙烯（PS）珠的分离8微米，25微米和人类皮肤微血管内皮细胞系（HMEC-1细胞）和人肝细胞系（HEPG2细胞）悬浮在DEP缓冲液中。我们表明，不仅可以实现高流速分离，而且可以在分离和聚焦之后，一个含有100％细胞的输出后获得高样品纯度。
Previous devices to separate cells by the characteristic force they experience due to dielectrophoresis, which depends on the size and electric properties of the particle, were limited by the flow rates and particle concentrations separation could be achieved at. To unlock the potential of Lab-on-a-chip technology to create flexible, efficient and multifunction devices at low cost it is necessary to increase the rates at which separations can be performed. Here we present a device capable of high throughput continuous separation of cells from microparticles and demonstrate separation at 7 uL/min in a microchannel with a high density of cells and microparticles in the sample of 10^8-10^9 cells/mL. This device uses the characteristic response of the particles due to dielectrophoresis to provide the initial separation before crucially focusing the particles into completely separate particle streams allowing the operation at high flow rates and particle concentrations. The device is demonstrated with the separation of polystyrene (PS) beads 8 microns, 25 microns and human dermal microvascular endothelial cell line (HMEC-1 cells) and human liver cell line (HepG2 cells) suspended in a DEP buffer solution. We show that it is possible to achieve not only high flow rate separation but also high sample purity with, after separation and focusing, one output containing 100% cells.
