论文标题
脉冲电磁场暴露对纳米 - $ tio_ {2} $表面培养的人间充质干细胞的骨诱导的影响
The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-$TiO_{2}$ surfaces
论文作者
论文摘要
人骨髓衍生的间充质干细胞（HBM-MSC）被认为是骨骼修复和再生的巨大希望。已经为揭示促进干细胞成骨分化的最佳策略而付出了巨大的努力。在先前的研究中，暴露于物理刺激的HBM-MSC（例如脉冲电磁场（PEMFS））或直接在纳米结构的钛表面（$ tio_ {2} $）上播种，以改善其在骨生成状态下与成核细胞的分化。在本研究中，研究了每日PEMF暴露对纳米结构的$ tio_ {2} $（簇低于100 nm的尺寸）的HBM-MSC的成骨分化的影响。 $ tio_ {2} $ - 种子细胞暴露于PEMF（磁场强度：2 mt；诱导电场的强度：5 mV；频率：75 Hz），并根据细胞生理修饰和成骨分化进行检查。 Results showed that PEMF exposure affected $TiO_{2}$-seeded cells osteogenesis by interfering with selective calcium-related osteogenic pathways, and greatly enhanced hBM-MSCs osteogenic features such as the expression of early/late osteogenic genes and protein production (e.g., ALP, COL-I, osteocalcin and osteopontin) and ALP activity.最后，与未处理的细胞培养物相比，经PEMF处理的细胞导致分泌到条件培养基量更高的BMP-2，DCN和COL-I。这些发现再次证实了PEMF的骨诱导潜力，这表明它与$ tio_ {2} $纳米结构的表面的结合可能是骨组织工程应用中的一个不错选择。
Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are considered a great promise in the repair and regeneration of bone. Considerable efforts have been oriented towards uncovering the best strategy to promote stem cells osteogenic differentiation. In previous studies, hBM-MSCs exposed to physical stimuli such as pulsed electromagnetic fields (PEMFs) or directly seeded on nanostructured titanium surfaces ($TiO_{2}$) were shown to improve their differentiation to osteoblasts in osteogenic condition. In the present study, the effect of a daily PEMF-exposure on osteogenic differentiation of hBM-MSCs seeded onto nanostructured $TiO_{2}$ (with clusters under 100 nm of dimension) was investigated. $TiO_{2}$-seeded cells were exposed to PEMF (magnetic field intensity: 2 mT; intensity of induced electric field: 5 mV; frequency: 75 Hz) and examined in terms of cell physiology modifications and osteogenic differentiation. Results showed that PEMF exposure affected $TiO_{2}$-seeded cells osteogenesis by interfering with selective calcium-related osteogenic pathways, and greatly enhanced hBM-MSCs osteogenic features such as the expression of early/late osteogenic genes and protein production (e.g., ALP, COL-I, osteocalcin and osteopontin) and ALP activity. Finally, PEMF-treated cells resulted to secrete into conditioned media higher amounts of BMP-2, DCN and COL-I than untreated cell cultures. These findings confirm once more the osteoinductive potential of PEMF, suggesting that its combination with $TiO_{2}$ nanostructured surface might be a great option in bone tissue engineering applications.
