论文标题
半实验蛋白结构测定和建模的质谱法
Mass spectrometry for semi-experimental protein structure determination and modeling
论文作者
论文摘要
蛋白质的结构对于其功能至关重要。通过实验或通过计算方法预测的蛋白质结构可以确定，但两种方法的组合也是可以的。在这里，首先给出了有关实验结构确定方法及其利弊的概述。然后，我们描述质谱如何用于半实验性整合蛋白结构的确定。我们审查方法论并描述支持这种集成蛋白质结构预测方法的软件程序，利用距离限制从质谱分支实验中获得
The structure of proteins is essential for its function. The determination of protein structures is possible by experimental or predicted by computational methods, but also a combination of both approaches is possible. Here, first an overview about experimental structure determination methods with their pros and cons is given. Then we describe how mass spectrometry is useful for semi-experimental integrative protein structure determination. We review the methodology and describe software programs supporting such integrated protein structure prediction approaches, making use of distance constraints got from mass spectrometry cross-linking experiments
