论文标题
基于频率选择性表面
Ultrasensitive THz Biosensor for PCR-free cDNA detection based on frequency selective surfaces
论文作者
论文摘要
THZ技术是无标签检测生物分子的强大工具。但是，在生物医学诊断中应用THZ传感器需要显着降低较低的检测极限。本文报告了一种基于非对称双分裂环谐振器（ADSRR）的超敏感生物传感器，用于在生理相关浓度下直接标记和无PCR检测DNA。我们引入选择性功能化和局部电场浓度，以增强ADSRR的敏感性和特异性。使用人类肿瘤标记MIA在没有PCR扩大的总RNA中产生的cDNA样品中证明了传感器特性。提出了浓度低至$ 1.55 \，\ times \，10^{ - 12} \，$ mol/l的DNA样品的测量值。
THz technologies are a powerful tool for label-free detection of biomolecules. However, significant reduction of the lower detection limit is required to apply THz-sensors in biomedical diagnosis. This paper reports an ultrasensitive THz-biosensor based on asymmetric double split ring resonators (aDSRR) for the direct label- and PCR-free detection of DNA at physiologically relevant concentrations. We introduce selective functionalization and localized electric field concentration to enhance aDSRR sensitivity and specificity. The sensor characteristics are demonstrated using the human tumor marker MIA in cDNA samples produced from total RNA without PCR-amplification. Measurements of DNA samples with concentrations as low as $1.55\,\times\,10^{-12}\,$mol/l are presented.
