论文标题
外部毛细胞宏观绘制中复杂的非线性电容：膜张力的影响
Complex nonlinear capacitance in outer hair cell macro-patches: effects of membrane tension
论文作者
论文摘要
外毛细胞（OHC）非线性电容（NLC）代表Prestin（SLC26A5）的电压传感器电荷运动，这是负责OHC电动性的蛋白质。 NLC频率响应的先前度量已采用了可能发生的方法，这些方法未评估介电损耗（传感器电荷与电压偏置）的影响，并且可以想象可能会影响Prestin的频率依赖性活动。在这里，我们评估Prestin的复杂电容至30 kHz，发现其使用这种方法确定的频率响应与所有先前的估计值一致。我们还表明，尽管其电压操作范围发生了很大的变化，但膜张力对普雷斯汀的频率响应没有影响，这表明普雷斯汀过渡速率的变化并不能解释这一变化。 Prestin活性跨越频率的幅度超过了由水杨酸盐处理引起的NLC的减少，而水杨酸盐处理已知被废除的耳蜗放大。因此，如果Prestin在一些哺乳动物处理的非常高的声学频率下，这种滚动必须限制有效性。
Outer hair cell (OHC) nonlinear capacitance (NLC) represents voltage sensor charge movements of prestin (SLC26a5), the protein responsible for OHC electromotility. Previous measures of NLC frequency response have employed methods which did not assess the influence of dielectric loss (sensor charge movements out of phase with voltage) that may occur, and such loss conceivably may influence the frequency dependent activity of prestin. Here we evaluate complex capacitance of prestin out to 30 kHz and find that its frequency response determined using this approach coincides with all previous estimates. We also show that membrane tension has no effect on the frequency response of prestin, despite substantial shifts in its voltage operating range, indicating that prestin transition rate alterations do not account for the shifts. The magnitude roll-off of prestin activity across frequency surpasses the reductions of NLC caused by salicylate treatments that are known to abolish cochlear amplification. Such roll-off must therefore limit the effectiveness if prestin in contributing to cochlear amplification at the very high acoustic frequencies processed by some mammals.
