论文标题
空间角荧光显微镜III。受约束的角扩散，极化激发和高NA成像
Spatio-angular fluorescence microscopy III. Constrained angular diffusion, polarized excitation, and high-NA imaging
论文作者
论文摘要
我们研究了荧光分子在角电位孔中的旋转扩散，这些扩散分子的激发和随后的排放，以及使用高NA平面光学显微镜进行这些排放的成像。尽管偶极子排放仅传输六个低频角组件，但我们表明，角结构化照明可以将高频成分的成分置于成像系统的通带中。我们表明，可测量的角成分的数量受三个时间尺度之间的关系限制：旋转扩散时间，荧光衰减时间和采集时间。我们通过在快速角扩散，慢角度扩散和弱电位的范围内模拟数值幻影来展示我们的模型。
We investigate rotational diffusion of fluorescent molecules in angular potential wells, the excitation and subsequent emissions from these diffusing molecules, and the imaging of these emissions with high-NA aplanatic optical microscopes. Although dipole emissions only transmit six low-frequency angular components, we show that angular structured illumination can alias higher frequency angular components into the passband of the imaging system. We show that the number of measurable angular components is limited by the relationships between three time scales: the rotational diffusion time, the fluorescence decay time, and the acquisition time. We demonstrate our model by simulating a numerical phantom in the limits of fast angular diffusion, slow angular diffusion, and weak potentials.
