论文标题

关于迁移上皮单层中速度,路段和细胞间应力之间的关系

On the relationship between velocities, tractions, and intercellular stresses in the migrating epithelial monolayer

论文作者

Green, Yoav, Fredberg, Jeffrey J., Butler, James P.

论文摘要

目前尚不清楚迁移上皮单层中的速度,文流和细胞间应力之间的关系。十年前,提出了一种称为单层应力显微镜(MSM)的方法,从该方法鉴于牵引场,可以从中计算细胞间应力。 MSM的核心假设是,单层内的细胞间应力与被动系统(如hookean固体(弹性板)或牛顿流体(薄流体膜))相似,这意味着位移/速度和轨道之间的关系。由于缺乏独立测量的细胞间应力,因此很难验证MSM。我们在这里提供的另一种方法是基于单层速度场和细胞/底物轨道的同时测量。在有限的假设中,速度场就足以计算文流,然后我们可以将其直接与牵引力显微镜测量的速度进行比较。我们发现计算出的文章和测量的街道是不相关的。由于经典MSM和对位移或速度和拖网之间关系的纯粹粘性描述取决于线性本构定律,因此需要对这些方法进行一些修改。一种可能的分辨率是包括活性力。为此,我们在活跃的力量密度与测得的速度(或位移)场之间提供了新的关系,而牛顿定律必须遵守这座遗迹。

The relationship between velocities, tractions, and intercellular stresses in the migrating epithelial monolayer are currently unknown. Ten years ago, a method known as Monolayer Stress Microscopy (MSM) was suggested from which the intercellular stresses could be computed given a traction field. The core assumption of MSM is that the intercellular stresses within the monolayer behave similarly to passive systems like a Hookean solid (an elastic sheet) or a Newtonian fluid (thin fluid film), implying a relation between the displacements/velocities and tractions. Due to the lack of independently measured intercellular stresses, validation of MSM is difficult. An alternative approach, which we give here, is based on simultaneous measurements of the monolayer velocity field and the cell/substrate tractions. With limited assumptions, the velocity field suffices to compute tractions, which we can then compare directly with those measured by traction force microscopy. We find that the calculated tractions and measured tractions are uncorrelated. Since both classical MSM and a purely viscous description of the relation between displacements or velocities and tractions depends on a linear constitutive law, it follows that some modification of these approaches is needed. One possible resolution is the inclusion of an active force. To this end, we give a new relationship between the active force density and the measured velocity(or displacement) field, and tractions, which by Newton's laws, must be obeyed.

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