ISO INTERNATIONAL STANDARD 22717 Second edition 2015-11-15 Cosmetics Microbiology Detection of Pseudomonas aeruginosa Cosmétiques- Microbiologie - Détection de Pseudomonas aeruginosa Reference number ISO 22717:2015(E) SO International Organization for Standardization @ IS0 2015 ZHEJIANG INST OF STANDARDIZATION C15956617 vided by IHS under itted without license from IHS IS0 22717:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS02015,PublishedinSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Intenationaibr Prganization for Standardization icensee-ZHEJIANG INST.OF STANDARDIzAoISQ s.5 -All rights reserved etworking permitted without license from IHS IS0 22717:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. .1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Diluents and culture media .2 5.1 General .2 5.2 Diluent for the bacterial suspension (tryptone sodium chloride solution) .3 5.2.1 General 5.2.2 Composition .3 5.2.3 Preparation. .3 5.3 Culture media. .3 5.3.1 .3 General. 5.3.2 Agar medium for the suitability test (see Clause 11) [soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA)) .3 5.3.3 Enrichment broth ..4 5.3.4 Selective agar medium for isolation of Pseudomonas aeruginosa .5 5.3.5 Selective agar medium for confirmation of Pseudomonas aeruginosa ..5 6 Apparatus and glassware .6 7 Strains of microorganisms ..6 8 Handling of cosmetic products and laboratory samples ..6 9 Procedure .6 9.1 Generalrecommendatior ..6 9.2 Preparation of the initial suspension in the enrichment broth ..6 9.2.1 General 9.2.2 Water-miscible products. .7 9.2.3 Water-immiscible products ..7 9.2.4 Filterable products .7 9.3 Incubation of the inoculated enrichment broth .7 9.4 Detection and Identification of Pseudomonas aeruginosa .7 9.4.1 Isolation .7 9.4.2 Identification of Pseudomonas aeruginosa .7 10 Expression of results (detection of Pseudomonas aeruginosa) ..8 11 Neutralization of the antimicrobial properties of the product. .8 11.1 General. ..8 11.2 Preparation of the inoculum ..8 11.3 Suitability of the detection method ..8 11.3.1 Procedure 11.3.2 Interpretation of suitability test results ..9 12 Test report ..9 Annex A (informative) Other enrichment broths .10 Annex B (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids .12 Bibliography ..13 iii e=ZHEJIANG INST OF STANDARDIZATION C1 5956617