ISO INTERNATIONAL STANDARD 18743 First edition 2015-09-15 Microbiology of the food chain Detection of Trichinella larvae in meat by artificial digestion method Microbiologie de la chaine alimentaire - Recherche des larves de Trichinella dans la viande par une méthode de digestion artificielle Reference number ISO 18743:2015(E) ISO International Organization for Standardization ee=Zhejang Institute of Standardization 5956617 @IS02015 vided by IHS under No reproduction or networking permitted without license from IHS IS0 18743:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2015, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Ch. de Blandonnet 8 · CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Internatinai br DrganizationforStandardization Not for Resale, 2015/11/11 08:23:24 networking permited withoutlicense from IHS IS0 18743:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions ..1 4 Principle .2 4.1 General .2 4.2 Sample size .2 4.3 Blending/grinding of the muscle sample .2 4.4 Preparation of the digest fluid .2 4.5 Digestion of the chopped meat 2 4.6 Filtration of the digest fluid .3 4.7 Sedimentation of the digest fluid .3 4.8 Microscopic examination ..3 4.9 Verification of findings .3 5 Reagents. ..3 6 Apparatus. ..4 7 Sampling, labelling, and transport ..5 8 Sample preparation. ..5 9 Procedure. .5 9.1 .5 General. 9.2 Blending/grinding 5 9.3 Preparation of the digest fluid. .6 9.4 Digestion of the chopped meat in the glass beaker .6 9.5 Filtration of the digest fluid .6 9.6 Sedimentation of the digest fluid in the separatory funnel 7 9.7 Collection of the primary and secondary sediment .7 9.8 Microscopic examination ..7 10 Documentation. ..8 11 Expression of the results ..8 12 Safety measures. ..8 Annex A (normative) Sample collection ..9 Annex B (normative) Frozen samples. ..11 Annex C (informative) Artificial digestion magnetic stirrer method ..12 Annex D (informative) Example of a laboratory worksheet for recording data when testing pooled samples by digestion assay ..14 Bibliography .15 ii icensee=Zhejiang Institute of Standardization 5956617 without license from IHS Not for Resale, 2015/11/11 08:23:24