ISO INTERNATIONAL STANDARD 18254-1 Firstedition 2016-04-15 Textiles Method for the detection and determination of alkylphenol ethoxylates (APEO) Part 1: Method using HPLC-MS Textiles - Méthode de détection et de détermination des alkylphenols éthoxylés (APEO) Partie 1:Méthode utilisant la CLHP-SM Reference number IS0 18254-1:2016(E) ISO International Organization for Standardization @ IS0 2016 HEJIANG INST OF STANDARDIZATION C1 5956617 ed without license from IHS IS0 18254-1:2016(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Intenationaibr PrganizationforStandardization nsee-ZHEJIANG INST OF STANDARDIZzARoISQ06 - All rights reserved etworking permitted without license from IHS IS0 18254-1:2016(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope.. ..1 2 Principle. ..1 3 Reagents ..1 4 Apparatus. .2 4.1 Apparatus and auxiliaries for preparing the sample .2 4.2 Chromatographic equipment .2 5 Procedure .2 5.1 Standard preparation .2 5.2 Sample preparation .3 5.3 Analysis. .3 6 Calculation of results .3 6.1 Determination of the R value for each congener of APEO .3 6.2 Calibration curve. .3 6.3 Calculation of concentration of APEO result .4 7 Test report ..4 Annex A (informative) Examples of chromatographic method - Lc/MS Annex B (informative) Examples of chromatographic method - Lc/MS/MS ..8 Bibliography .10 iii =ZHEJIANG INSTOF STANDARDIZATION C15956617 thout license from IHS