ISO INTERNATIONAL STANDARD 16198 First edition 2015-01-15 Soil quality Plant-based test to assess the environmental bioavailability of trace elements to plants Qualité du sol - Test vegetal pour I'évaluation de la biodisponibilité environnementale des élements traces pour les végeétaux Reference number ISO 16198:2015(E) ISO IS02015 IS0 16198:2015(E) COPYRIGHTPROTECTEDDOCUMENT @IS02015 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or Iso's member body in the country of the requester. ISO copyright office Case postale 56 · CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax+41227490947 E-mail [email protected] Web www.iso.org Published in Switzerland ii @ IS0 2015 - All rights reserved IS0 16198:2015(E) Contents Page Foreword ..iv Introduction. .V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions .2 4 Principle ..3 5 Laboratory apparatus ..5 6 Reagents ..5 6.1 General ..5 7 Biological and growing apparatus .6 7.1 Plant species ..6 7.2 Biotest apparatus. ..6 7.3 Composition of the nutrient solutions .9 7.4 Climatic conditions in the growth chamber .10 8 Pre-treatment and analysis of soil or soil material sample ..10 8.1 Sample size and particle size reduction .10 8.2 Analyses.. .10 9 Experimental and analytical procedure ..11 9.1 Overview of the procedure ..11 9.2 Selection and preparation of seeds .11 9.3 Preculture period: Germination and pre-growth in hydroponics. .11 9.4 Preparation and incubation of soil or soil material ..12 9.5 Test culture period: Plant growth in contact with soil or soil material 12 9.6 Plantharvests .13 9.7 Grinding and digestion of shoots and roots ..13 9.8 Analyticaldetermination ..14 10 Validity criteria ..14 11 Assessment of the results ..14 11.1 Determination of trace element concentrations and uptake flux in plants .14 11.2 Data presentation. ..16 11.3 Expression of the results ..16 12 Statistical analysis .16 12.1 General ..16 12.2 Plant biomasses ..16 12.3 Bioavailability end points ..17 13 Test report ..17 Annex A (informative) Plant species adapted to the biotest procedure ..19 Annex B (informative) Technical drawings of the different components of the biotest .21 Annex C (informative) Seed selection and seed density in plant pot for a range of species tested with the standardized experimental procedure .24 Annex D (informative) Digestion and analysis of plant samples .26 Annex E (informative) Range of biomasses and trace element quantities in control plant pots....28 Annex F (informative) Ring-test .29 Bibliography ..43 snSandardizan rights reserved ii